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rabbit polyclonal anti pstat1 igg  (New England Biolabs)


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    New England Biolabs rabbit polyclonal anti pstat1 igg
    In 1A , IFNAR1 and IFNAR2 expression was analyzed in the CD3 − CD19 + CD27 + and CD3 − CD19 + CD27 − lymphocyte gates. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI) of IFNAR1 and IFNAR2 after substraction of MFI values obtained in isotype control in naive (CD27 − ) and memory (CD27 + ) B cell subsets. The results correspond to the mean ± SEM of the values obtained with cells from 6 healthy donors. 1B shows the purity of B cell preparations (see methods). In 1C , purified B cells were activated with IFN-α for 1 h then lysed. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT2, and the membranes were reprobed with anti-STAT2. The data shown in 1C are representative of 2 independent experiments. 1D, left panel : B cells were activated for 1 hour with IFN-α. Western blotting was performed on whole-cell lysates by using <t>anti-phospho-STAT1</t> or anti-phospho-STAT4. The membranes were then reprobed with anti-STAT1 or anti-STAT4. The data shown in 1D , left panel are representative of 2 independent experiments. 1D, right panel : B cells were activated with IFN-α for 1 hour or left untreated. They were then fixed, permeabilized, and stained with anti-STAT4 or anti-STAT1 (green) plus propidium iodide (nuclear staining, red). Nuclear translocation was examined by confocal microscopy. Yellow spots indicate nuclear STAT. Similar results were obtained in three other experiments. In 1E , the kinetics of STAT1 and STAT4 phosphorylation was analyzed in B cells by flow cytometry with phospho-STAT-specific antibodies. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI), after subtraction of MFI values obtained in isotype controls, of phospho-STAT1 and phospho-STAT4 in B cells. The data shown in 1E are the mean ± SEM for cells from 4 healthy donors.
    Rabbit Polyclonal Anti Pstat1 Igg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+pstat1+igg/pmc03084831-108-38-42
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal anti pstat1 igg - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Interferon-Alpha Triggers B Cell Effector 1 (Be1) Commitment"

    Article Title: Interferon-Alpha Triggers B Cell Effector 1 (Be1) Commitment

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0019366

    In 1A , IFNAR1 and IFNAR2 expression was analyzed in the CD3 − CD19 + CD27 + and CD3 − CD19 + CD27 − lymphocyte gates. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI) of IFNAR1 and IFNAR2 after substraction of MFI values obtained in isotype control in naive (CD27 − ) and memory (CD27 + ) B cell subsets. The results correspond to the mean ± SEM of the values obtained with cells from 6 healthy donors. 1B shows the purity of B cell preparations (see methods). In 1C , purified B cells were activated with IFN-α for 1 h then lysed. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT2, and the membranes were reprobed with anti-STAT2. The data shown in 1C are representative of 2 independent experiments. 1D, left panel : B cells were activated for 1 hour with IFN-α. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT1 or anti-phospho-STAT4. The membranes were then reprobed with anti-STAT1 or anti-STAT4. The data shown in 1D , left panel are representative of 2 independent experiments. 1D, right panel : B cells were activated with IFN-α for 1 hour or left untreated. They were then fixed, permeabilized, and stained with anti-STAT4 or anti-STAT1 (green) plus propidium iodide (nuclear staining, red). Nuclear translocation was examined by confocal microscopy. Yellow spots indicate nuclear STAT. Similar results were obtained in three other experiments. In 1E , the kinetics of STAT1 and STAT4 phosphorylation was analyzed in B cells by flow cytometry with phospho-STAT-specific antibodies. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI), after subtraction of MFI values obtained in isotype controls, of phospho-STAT1 and phospho-STAT4 in B cells. The data shown in 1E are the mean ± SEM for cells from 4 healthy donors.
    Figure Legend Snippet: In 1A , IFNAR1 and IFNAR2 expression was analyzed in the CD3 − CD19 + CD27 + and CD3 − CD19 + CD27 − lymphocyte gates. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI) of IFNAR1 and IFNAR2 after substraction of MFI values obtained in isotype control in naive (CD27 − ) and memory (CD27 + ) B cell subsets. The results correspond to the mean ± SEM of the values obtained with cells from 6 healthy donors. 1B shows the purity of B cell preparations (see methods). In 1C , purified B cells were activated with IFN-α for 1 h then lysed. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT2, and the membranes were reprobed with anti-STAT2. The data shown in 1C are representative of 2 independent experiments. 1D, left panel : B cells were activated for 1 hour with IFN-α. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT1 or anti-phospho-STAT4. The membranes were then reprobed with anti-STAT1 or anti-STAT4. The data shown in 1D , left panel are representative of 2 independent experiments. 1D, right panel : B cells were activated with IFN-α for 1 hour or left untreated. They were then fixed, permeabilized, and stained with anti-STAT4 or anti-STAT1 (green) plus propidium iodide (nuclear staining, red). Nuclear translocation was examined by confocal microscopy. Yellow spots indicate nuclear STAT. Similar results were obtained in three other experiments. In 1E , the kinetics of STAT1 and STAT4 phosphorylation was analyzed in B cells by flow cytometry with phospho-STAT-specific antibodies. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI), after subtraction of MFI values obtained in isotype controls, of phospho-STAT1 and phospho-STAT4 in B cells. The data shown in 1E are the mean ± SEM for cells from 4 healthy donors.

    Techniques Used: Expressing, Staining, Fluorescence, Purification, Western Blot, Translocation Assay, Confocal Microscopy, Flow Cytometry

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Interferon-Alpha Triggers B Cell Effector 1 (Be1) Commitment
    Article Snippet: They were then washed with PBS containing 50 μM sodium orthovanadate and resuspended in lysis buffer with a protease inhibitor cocktail (Roche Diagnostics). .. Proteins were fractionated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in reducing conditions, then transferred to polyvinylidene difluoride filters (PVDF, Boehringer Mannheim) and probed with rabbit polyclonal anti-pSTAT4 IgG (Zymed), rabbit polyclonal anti-pSTAT2 IgG (Upstate Biotechnology) or rabbit polyclonal anti-pSTAT1 IgG (New England Biolabs), followed by the secondary antibody (horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit IgG (Jackson Immunoresearch). .. PVDF blots were developed with SuperSignal WestPico kits (Pierce).



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    rabbit polyclonal anti pstat1 igg  (New England Biolabs)
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    New England Biolabs rabbit polyclonal anti pstat1 igg
    In 1A , IFNAR1 and IFNAR2 expression was analyzed in the CD3 − CD19 + CD27 + and CD3 − CD19 + CD27 − lymphocyte gates. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI) of IFNAR1 and IFNAR2 after substraction of MFI values obtained in isotype control in naive (CD27 − ) and memory (CD27 + ) B cell subsets. The results correspond to the mean ± SEM of the values obtained with cells from 6 healthy donors. 1B shows the purity of B cell preparations (see methods). In 1C , purified B cells were activated with IFN-α for 1 h then lysed. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT2, and the membranes were reprobed with anti-STAT2. The data shown in 1C are representative of 2 independent experiments. 1D, left panel : B cells were activated for 1 hour with IFN-α. Western blotting was performed on whole-cell lysates by using <t>anti-phospho-STAT1</t> or anti-phospho-STAT4. The membranes were then reprobed with anti-STAT1 or anti-STAT4. The data shown in 1D , left panel are representative of 2 independent experiments. 1D, right panel : B cells were activated with IFN-α for 1 hour or left untreated. They were then fixed, permeabilized, and stained with anti-STAT4 or anti-STAT1 (green) plus propidium iodide (nuclear staining, red). Nuclear translocation was examined by confocal microscopy. Yellow spots indicate nuclear STAT. Similar results were obtained in three other experiments. In 1E , the kinetics of STAT1 and STAT4 phosphorylation was analyzed in B cells by flow cytometry with phospho-STAT-specific antibodies. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI), after subtraction of MFI values obtained in isotype controls, of phospho-STAT1 and phospho-STAT4 in B cells. The data shown in 1E are the mean ± SEM for cells from 4 healthy donors.
    Rabbit Polyclonal Anti Pstat1 Igg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+pstat1+igg/pmc03084831-108-38-42
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal anti pstat1 igg - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    In 1A , IFNAR1 and IFNAR2 expression was analyzed in the CD3 − CD19 + CD27 + and CD3 − CD19 + CD27 − lymphocyte gates. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI) of IFNAR1 and IFNAR2 after substraction of MFI values obtained in isotype control in naive (CD27 − ) and memory (CD27 + ) B cell subsets. The results correspond to the mean ± SEM of the values obtained with cells from 6 healthy donors. 1B shows the purity of B cell preparations (see methods). In 1C , purified B cells were activated with IFN-α for 1 h then lysed. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT2, and the membranes were reprobed with anti-STAT2. The data shown in 1C are representative of 2 independent experiments. 1D, left panel : B cells were activated for 1 hour with IFN-α. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT1 or anti-phospho-STAT4. The membranes were then reprobed with anti-STAT1 or anti-STAT4. The data shown in 1D , left panel are representative of 2 independent experiments. 1D, right panel : B cells were activated with IFN-α for 1 hour or left untreated. They were then fixed, permeabilized, and stained with anti-STAT4 or anti-STAT1 (green) plus propidium iodide (nuclear staining, red). Nuclear translocation was examined by confocal microscopy. Yellow spots indicate nuclear STAT. Similar results were obtained in three other experiments. In 1E , the kinetics of STAT1 and STAT4 phosphorylation was analyzed in B cells by flow cytometry with phospho-STAT-specific antibodies. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI), after subtraction of MFI values obtained in isotype controls, of phospho-STAT1 and phospho-STAT4 in B cells. The data shown in 1E are the mean ± SEM for cells from 4 healthy donors.

    Journal: PLoS ONE

    Article Title: Interferon-Alpha Triggers B Cell Effector 1 (Be1) Commitment

    doi: 10.1371/journal.pone.0019366

    Figure Lengend Snippet: In 1A , IFNAR1 and IFNAR2 expression was analyzed in the CD3 − CD19 + CD27 + and CD3 − CD19 + CD27 − lymphocyte gates. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI) of IFNAR1 and IFNAR2 after substraction of MFI values obtained in isotype control in naive (CD27 − ) and memory (CD27 + ) B cell subsets. The results correspond to the mean ± SEM of the values obtained with cells from 6 healthy donors. 1B shows the purity of B cell preparations (see methods). In 1C , purified B cells were activated with IFN-α for 1 h then lysed. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT2, and the membranes were reprobed with anti-STAT2. The data shown in 1C are representative of 2 independent experiments. 1D, left panel : B cells were activated for 1 hour with IFN-α. Western blotting was performed on whole-cell lysates by using anti-phospho-STAT1 or anti-phospho-STAT4. The membranes were then reprobed with anti-STAT1 or anti-STAT4. The data shown in 1D , left panel are representative of 2 independent experiments. 1D, right panel : B cells were activated with IFN-α for 1 hour or left untreated. They were then fixed, permeabilized, and stained with anti-STAT4 or anti-STAT1 (green) plus propidium iodide (nuclear staining, red). Nuclear translocation was examined by confocal microscopy. Yellow spots indicate nuclear STAT. Similar results were obtained in three other experiments. In 1E , the kinetics of STAT1 and STAT4 phosphorylation was analyzed in B cells by flow cytometry with phospho-STAT-specific antibodies. A representative staining profile is shown in the left-hand graph. The right-hand graph represents corrected mean fluorescence intensities (MFI), after subtraction of MFI values obtained in isotype controls, of phospho-STAT1 and phospho-STAT4 in B cells. The data shown in 1E are the mean ± SEM for cells from 4 healthy donors.

    Article Snippet: Proteins were fractionated by 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in reducing conditions, then transferred to polyvinylidene difluoride filters (PVDF, Boehringer Mannheim) and probed with rabbit polyclonal anti-pSTAT4 IgG (Zymed), rabbit polyclonal anti-pSTAT2 IgG (Upstate Biotechnology) or rabbit polyclonal anti-pSTAT1 IgG (New England Biolabs), followed by the secondary antibody (horseradish peroxidase (HRP)-conjugated goat anti-mouse or anti-rabbit IgG (Jackson Immunoresearch).

    Techniques: Expressing, Staining, Fluorescence, Purification, Western Blot, Translocation Assay, Confocal Microscopy, Flow Cytometry